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KMID : 1134820000290061112
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Journal of the Korean Society of Food Science and Nutrition
2000 Volume.29 No. 6 p.1112 ~ p.1115
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The Mode of Antimicrobial Mechanism of Aristolochia contorta Bge. Extract
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Lee In-Eun
Cho Sung-Hwan
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Abstract
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To develop natural antimicrobial agents for keeping qualities of agricultural products and their process- ing foods, the antimiocrobial actions of Aristolochia contorta Bge. extract, which showed remarkable anti- microbial effects against microorganisms causing their decay, were investigated. Direct visualization of microbial cells by using both transmission electron microscope and scanning electron microscope showed that microbial cell membrane was destroyed by treating with the dilute extract solution. This change of cellular membrane permeability could be identified in the experiment that 0-nitrophenyl-β-D -galactopyranoside (ONPG), the artificial substrate of β-galactosidase, was hydrolyzed in the presence of the extract, indicating that the membrane was perturbed. Consequently, the functionality of Aristolochia contorta Bge. extract as antimicrobial agents for keeping qualities of agricultural products and their processing foods may be recommended.
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KEYWORD
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natural antimicrobial agents, transmission electron microscope, scanning electron microscope
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